Interactions between bacterial surface and nanoparticles govern the performance of "chemical nose" biosensors.

نویسندگان

  • Mohit S Verma
  • Shih-Chung Wei
  • Jacob L Rogowski
  • Jackson M Tsuji
  • Paul Z Chen
  • Chii-Wann Lin
  • Lyndon Jones
  • Frank X Gu
چکیده

Rapid and portable diagnosis of pathogenic bacteria can save lives lost from infectious diseases. Biosensors based on a "chemical nose" approach are attracting interest because they are versatile but the governing interactions between bacteria and the biosensors are poorly understood. Here, we use a "chemical nose" biosensor based on gold nanoparticles to explore the role of extracellular polymeric substances in bacteria-nanoparticle interactions. We employ simulations using Maxwell-Garnett theory to show how the type and extent of aggregation of nanoparticles influence their colorimetric response to bacteria. Using eight different species of Gram-positive and Gram-negative bacteria, we demonstrate that this "chemical nose" can detect and identify bacteria over two orders of magnitude of concentration (89% accuracy). Additionally, the "chemical nose" differentiates between binary and tertiary mixtures of the three most common hospital-isolated pathogens: Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa (100% accuracy). We demonstrate that the complex interactions between nanoparticles and bacterial surface determine the colorimetric response of gold nanoparticles and thus, govern the performance of "chemical nose" biosensors.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

High performance liquid chromatographic analysis of reduction products of a thiolated DNA for immobilization on gold nanoparticles

DNA-based nano-biosensors are emerging scope in the field of biosensors. Many synthetic single stranded functional DNAs have been applied for development of such sensors, recently. Immobilization of DNA oligonucleotides on the surface of gold nanoparticles is a key step in the development of most colorimetric nano-biosensors. The bound DNA is usually thiolated and forms Au-S covalent bond to th...

متن کامل

Biological Applications of Bacterial Nano-Surface Layers : A Brief Overview

Surface layer as the outer protective coverage of bacteria and archaea are two-dimensional crystalline and symmetrical arrays of proteins that recently attract a lot of attention for biologist scientists. The surface layers of bacteria are usually 5 to 10 nm in diameter and represent highly porous protein lattices with uniform size and morphology with the pore sizes of 2 to 8 nm. The crucial an...

متن کامل

Mesoporous Silica Nanoparticles as a Nanocarrier for Delivery of Vitamin C

Background: In the last decades, mesoporous silica nanoparticles (MSN) are improved for drug delivery, imaging, and biomedical applications due to their special properties such as large surface area, high drug loading capacity, tunable pore size, and modification of surface area by functional groups. Objectives: The aim of this study was to evaluate MSNs as carriers for oral colon-specific and...

متن کامل

Colorimetric gold nanoparticles-based aptasensors

Recognition of different agents including chemical and biological plays important role in forensic, biomedical and environmentalfield.In recent decades, nanotechnology and nano materials had a high impact on development of sensors. Using  nanomaterials in construction of biosensors can effectively improve the Sensitivity and other features of biosensors. Different type of nanostructures includi...

متن کامل

Investigation the effect of nanocomposite material on permeation flux of polyerthersulfone membrane using a mathematical approach

Integrally skinned asymmetric membranes based on nanocompositepolyethersulfone were prepared by the phase separation process using the supercritical CO2 as a nonsolvent for the polymer solution. In present study, the effects of temperature and nanoparticle on selectivity performance and permeability of gases has beeninvestigated. It is shown that the presence of silica nanoparticles not only di...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Biosensors & bioelectronics

دوره 83  شماره 

صفحات  -

تاریخ انتشار 2016